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Walter and Eliza Hall Institute Research Data Access Committee

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Ian Majewski
dataaccess@wehi.edu.au

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This DAC controls 14 datasets

Dataset ID	Description	Technology	Samples
EGAD00001011271	Whole genome sequencing on DNA from snap frozen tumour sample and matched whole blood of patient #130. Analysis used the QIAamp DNA Mini Kit. Libraries were prepared using the Illumina TruSeq Nano library method using 200ng of DNA. Extracted DNA was sheared using the Covaris M220 Focused-ultrasonicator with a target fragment length of 550bp through bead size selection. The libraries were sequenced at depth of 40x for germline DNA and 80-100x for tumour DNA using paired 150bp reads.	Illumina NovaSeq 6000	2
EGAD00001011272	Whole Exome Sequencing of blood and FFPE tumour of patient #368.  150–300 ng of DNA was fragmented to approximately 200 bp using a focal acoustic device. Libraries were prepared with the Kapa Hyper Prep Kit and SureSelectXT adaptors. Hybridisation capture was performed with SureSelect Clinical Research Exome V2 baits following the SureSelectXT recommended protocol (Agilent). Indexed libraries were sequenced on an Illumina NovaSeq 6000 to generate paired-end 150 bp reads with average of 70-fold base coverage for the germline sample and 330-fold coverage for tumour sample.	Illumina NovaSeq 6000	2
EGAD50000000032	RNAseq for #1049, #111, #1217, #206, COV362	Illumina NovaSeq 6000	5
EGAD50000000033	WGS of tumour PDX and matched patient blood	Illumina NovaSeq 6000	5
EGAD50000000034	Exome sequencing of PDX and matched patient blood	NextSeq 500	4
EGAD50000000035	BROCA panel sequencing using the BROCA-HR v8 and BROCA-GO v1 versions of the gene panel	Illumina HiSeq 2500 Illumina NovaSeq 6000	15
EGAD50000000116	RNAseq for #111, #1177, #206, #201, #29, #931, WO-19, WO-2	Illumina NovaSeq 6000	8
EGAD50000000189	exon 11 mutated UWB1.289 and COV362 cell lines	Illumina NovaSeq 6000	2
EGAD50000000215	Exome sequencing of tumour PDX and matched patient blood	Illumina NovaSeq 6000	10
EGAD50000000216	Examination of Sample Multiplexing Reagents for Single Cell RNA-Seq. Nine techniques applied to samples from four PDX models: #105, #177, #233, #264	NextSeq 2000	9
EGAD50000000217	Whole genome sequencing of patient tumour and blood	Illumina NovaSeq 6000	2
EGAD50000000270	Targeted sequencing using the TruSight Oncology 500 DNA probes panel, on samples 162 and 521	NextSeq 500	4
EGAD50000000271	TruSight Oncology 500 RNA probes panel on case 521	NextSeq 500	2
EGAD50000000272	RNA extracted from FFPE tumour samples 368, 455, 503, and 521, sequenced using the TruSight RNA Fusion Panel	NextSeq 500	4