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Extensive three-dimensional intratumor proteomic heterogeneity revealed by multiregion sampling in high-grade serous ovarian tumor specimens

Enriched tumor epithelium, tumor-associated stroma, and whole tissue were collected by laser microdissection from thin sections across spatially separated levels of ten high-grade serous ovarian carcinomas (HGSOCs) and analyzed by mass spectrometry, reverse phase protein arrays, and RNA sequencing. Unsupervised analyses of protein abundance data revealed independent clustering of an enriched stroma and enriched tumor epithelium, with whole tumor tissue clustering driven by overall tumor “purity.” Comparing these data to previously defined prognostic HGSOC molecular subtypes revealed protein and transcript expression from tumor epithelium correlated with the differentiated subtype, whereas stromal proteins (and transcripts) correlated with the mesenchymal subtype. Protein and transcript abundance in the tumor epithelium and stroma exhibited decreased correlation in samples collected just hundreds of microns apart. These data reveal substantial tumor microenvironment protein heterogeneity that directly bears on prognostic signatures, biomarker discovery, and cancer pathophysiology and underscore the need to enrich cellular subpopulations for expression profiling.

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DAC for "Extensive three-dimensional intratumor proteomic heterogeneity revealed by multiregion sampling in high-grade serous ovarian tumor specimens" by Hunt et al.

DATA ACCESS AGREEMENT These terms and conditions govern access to the managed access datasets (details of which are set out in Appendix I) to which the User Institution has requested access. The User Institution agrees to be bound by these terms and conditions. Definitions Authorised Personnel: The individuals at the User Institution to whom Women’s Health Integrated Research Center Data Access Committee grants access to the Data. This includes the User, the individuals listed in Appendix II and any other individuals for whom the User Institution subsequently requests access to the Data. Details of the initial Authorised Personnel are set out in Appendix II. Data: The managed access datasets to which the User Institution has requested access. Data Producers: Women’s Health Integrated Research Center Data Access Committee and the collaborators listed in Appendix I responsible for the development, organisation, and oversight of these Data. External Collaborator: A collaborator of the User, working for an institution other than the User Institution. Project: The project for which the User Institution has requested access to these Data. A description of the Project is set out in Appendix II. Publications: Includes, without limitation, articles published in print journals, electronic journals, reviews, books, posters and other written and verbal presentations of research. Research Participant: An individual whose data form part of these Data. Research Purposes: Shall mean research that is seeking to advance the understanding of genetics and genomics, including the treatment of disorders, and work on statistical methods that may be applied to such research. User: The principal investigator for the Project. User Institution(s): The Institution that has requested access to the Data. Women’s Health Integrated Research Center Data Access Committee: Your Institution details here 1. The User Institution agrees to only use these Data for the purpose of the Project (described in Appendix II) and only for Research Purposes. The User Institution further agrees that it will only use these Data for Research Purposes which are within the limitations (if any) set out in Appendix I. 2. The User Institution agrees to preserve, at all times, the confidentiality of these Data. In particular, it undertakes not to use, or attempt to use these Data to compromise or otherwise infringe the confidentiality of information on Research Participants. Without prejudice to the generality of the foregoing, the User Institution agrees to use at least the measures set out in Appendix I to protect these Data. 3. The User Institution agrees to protect the confidentiality of Research Participants in any research papers or publications that they prepare by taking all reasonable care to limit the possibility of identification. 4. The User Institution agrees not to link or combine these Data to other information or archived data available in a way that could re-identify the Research Participants, even if access to that data has been formally granted to the User Institution or is freely available without restriction. 5. The User Institution agrees only to transfer or disclose these Data, in whole or part, or any material derived from these Data, to the Authorised Personnel. Should the User Institution wish to share these Data with an External Collaborator, the External Collaborator must complete a separate application for access to these Data. 6. The User Institution agrees that the Data Producers, and all other parties involved in the creation, funding or protection of these Data: a) make no warranty or representation, express or implied as to the accuracy, quality or comprehensiveness of these Data; b) exclude to the fullest extent permitted by law all liability for actions, claims, proceedings, demands, losses (including but not limited to loss of profit), costs, awards damages and payments made by the Recipient that may arise (whether directly or indirectly) in any way whatsoever from the Recipient’s use of these Data or from the unavailability of, or break in access to, these Data for whatever reason and; c) bear no responsibility for the further analysis or interpretation of these Data. 7. The User Institution agrees to follow the Fort Lauderdale Guidelines (http://www.wellcome.ac.uk/stellent/groups/corporatesite/@policy_communications/documents/web_document/wtd003207.pdf ) and the Toronto Statement (http://www.nature.com/nature/journal/v461/n7261/full/461168a.html). This includes but is not limited to recognising the contribution of the Data Producers and including a proper acknowledgement in all reports or publications resulting from the use of these Data. 8. The User Institution agrees to follow the Publication Policy in Appendix III. This includes respecting the moratorium period for the Data Producers to publish the first peer-reviewed report describing and analysing these Data. 9. The User Institution agrees not to make intellectual property claims on these Data and not to use intellectual property protection in ways that would prevent or block access to, or use of, any element of these Data, or conclusion drawn directly from these Data. 10. The User Institution can elect to perform further research that would add intellectual and resource capital to these data and decide to obtain intellectual property rights on these downstream discoveries. In this case, the User Institution agrees to implement licensing policies that will not obstruct further research and to follow the U.S. National Institutes of Health Best Practices for the Licensing of Genomic Inventions (2005) (https://www.icgc.org/files/daco/NIH_BestPracticesLicensingGenomicInventions_2005_en.pdf ) in conformity with the Organisation for Economic Co-operation and Development Guidelines for the Licensing of the Genetic Inventions (2006) (http://www.oecd.org/science/biotech/36198812.pdf ). 11. The User Institution agrees to destroy/discard the Data held, once it is no longer used for the Project, unless obliged to retain the data for archival purposes in conformity with audit or legal requirements. 12. The User Institution will notify Women’s Health Integrated Research Center Data Access Committee within 30 days of any changes or departures of Authorised Personnel. 13. The User Institution will notify Women’s Health Integrated Research Center Data Access Committee prior to any significant changes to the protocol for the Project. 14. The User Institution will notify Women’s Health Integrated Research Center Data Access Committee as soon as it becomes aware of a breach of the terms or conditions of this agreement. 15. Women’s Health Integrated Research Center Data Access Committee may terminate this agreement by written notice to the User Institution. If this agreement terminates for any reason, the User Institution will be required to destroy any Data held, including copies and backup copies. This clause does not prevent the User Institution from retaining these data for archival purpose in conformity with audit or legal requirements. 16. The User Institution accepts that it may be necessary for the Data Producers to alter the terms of this agreement from time to time. As an example, this may include specific provisions relating to the Data required by Data Producers other than Women’s Health Integrated Research Center Data Access Committee. In the event that changes are required, the Data Producers or their appointed agent will contact the User Institution to inform it of the changes and the User Institution may elect to accept the changes or terminate the agreement. 17. If requested, the User Institution will allow data security and management documentation to be inspected to verify that it is complying with the terms of this agreement. 18. The User Institution agrees to distribute a copy of these terms to the Authorised Personnel. The User Institution will procure that the Authorised Personnel comply with the terms of this agreement. 19. This agreement (and any dispute, controversy, proceedings or claim of whatever nature arising out of this agreement or its formation) shall be construed, interpreted and governed by the laws of England and Wales and shall be subject to the exclusive jurisdiction of the English courts. Agreed for User Institution Signature: Name: Title: Date: Principal Investigator I confirm that I have read and understood this Agreement. Signature: Name: Title: Date: Agreed for Women’s Health Integrated Research Center Data Access Committee Signature: Name: Title: Date: APPENDIX I – DATASET DETAILS APPENDIX II ––PROJECT DETAILS APPENDIX III –– PUBLICATION POLICY APPENDIX I – DATASET DETAILS (to be completed by the data producer before passing to applicant) Dataset reference (EGA Study ID and Dataset Details) Name of project that created the dataset Extensive three-dimensional intratumor proteomic heterogeneity revealed by multiregion sampling in high-grade serous ovarian tumor specimens Names of other data producers/collaborators Allison L Hunt, Nicholas W Bateman, Waleed Barakat, Sasha Makohon-Moore, Brian L Hood, Kelly A Conrads, Ming Zhou, Valerie Calvert, Mariaelena Pierobon, Jeremy Loffredo, Tracy J Litzi, Julie Oliver, Dave Mitchell, Glenn Gist, Christine Rojas, Brian Blanton, Emma L Robinson, Kunle Odunsi, Anil K Sood, Yovanni Casablanca, Kathleen M Darcy, Craig D Shriver, Emanuel F Petricoin, Uma N M Rao, G Larry Maxwell, Thomas P Conrads Specific limitations on areas of research The Women’s Health Integrated Research Center Data Access Committee confirms that there are no limitations on areas of research for access and/or usage of this data. Minimum protection measures required The Women’s Health Integrated Research Center Data Access Committee confirms that this data is fully deidentified of all protected health information (PHI) and does not require additional protection measures for access and/or usage. File access: Data can be held in unencrypted files on an institutional compute system, with Unix user group read/write access for one or more appropriate groups but not Unix world read/write access behind a secure firewall. Laptops holding these data should have password protected logins and screenlocks (set to lock after 5 min of inactivity). If held on USB keys or other portable hard drives, the data must be encrypted. APPENDIX II – PROJECT DETAILS (to be completed by the Requestor) Details of dataset requested i.e., EGA Study and Dataset Accession Number Brief abstract of the Project in which the Data will be used (500 words max) All Individuals who the User Institution to be named as registered users Name of Registered User Email Job Title Supervisor* All Individuals that should have an account created at the EGA Name of Registered User Email Job Title APPENDIX III – PUBLICATION POLICY The Women’s Health Integrated Research Center Data Access Committee has provided written consent confirming no necessity for a publication moratorium using this data.

Studies are experimental investigations of a particular phenomenon, e.g., case-control studies on a particular trait or cancer research projects reporting matching cancer normal genomes from patients.

Study ID Study Title Study Type
EGAS00001005786 Other

This table displays only public information pertaining to the files in the dataset. If you wish to access this dataset, please submit a request. If you already have access to these data files, please consult the download documentation.

ID File Type Size Located in
EGAF00005709046 fastq.gz 1.5 GB
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EGAF00005709048 fastq.gz 1.5 GB
EGAF00005709049 fastq.gz 1.5 GB
EGAF00005709050 fastq.gz 1.4 GB
EGAF00005709051 fastq.gz 1.3 GB
EGAF00005709052 fastq.gz 1.3 GB
EGAF00005709053 fastq.gz 1.3 GB
EGAF00005709054 fastq.gz 1.6 GB
EGAF00005709055 fastq.gz 1.9 GB
EGAF00005709056 fastq.gz 1.4 GB
EGAF00005709057 fastq.gz 1.3 GB
EGAF00005709058 fastq.gz 2.3 GB
EGAF00005709059 fastq.gz 1.9 GB
EGAF00005709060 fastq.gz 1.2 GB
EGAF00005709061 fastq.gz 1.2 GB
EGAF00005709062 fastq.gz 1.3 GB
EGAF00005709063 fastq.gz 1.4 GB
EGAF00005709064 fastq.gz 1.6 GB
EGAF00005709065 fastq.gz 1.5 GB
EGAF00005709066 fastq.gz 1.5 GB
EGAF00005709067 fastq.gz 1.2 GB
EGAF00005709068 fastq.gz 1.4 GB
EGAF00005709069 fastq.gz 1.8 GB
EGAF00005709070 fastq.gz 1.6 GB
EGAF00005709071 fastq.gz 1.4 GB
EGAF00005709072 fastq.gz 1.5 GB
EGAF00005709073 fastq.gz 1.5 GB
EGAF00005709074 fastq.gz 830.4 MB
EGAF00005709075 fastq.gz 886.8 MB
EGAF00005709076 fastq.gz 549.9 MB
EGAF00005709077 fastq.gz 945.5 MB
EGAF00005709078 fastq.gz 1.4 GB
EGAF00005709079 fastq.gz 1.5 GB
EGAF00005709080 fastq.gz 707.1 MB
EGAF00005709081 fastq.gz 1.7 GB
EGAF00005709082 fastq.gz 1.4 GB
EGAF00005709083 fastq.gz 1.9 GB
EGAF00005709084 fastq.gz 2.0 GB
EGAF00005709085 fastq.gz 908.7 MB
EGAF00005709086 fastq.gz 207.4 MB
EGAF00005709087 fastq.gz 1.1 GB
EGAF00005709088 fastq.gz 1.6 GB
EGAF00005709089 fastq.gz 1.4 GB
EGAF00005709090 fastq.gz 1.3 GB
EGAF00005709091 fastq.gz 1.5 GB
EGAF00005709092 fastq.gz 1.3 GB
EGAF00005709093 fastq.gz 894.2 MB
EGAF00005709094 fastq.gz 947.9 MB
49 Files (66.1 GB)