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Influence of the Microbiome on Epigenetic Mechanisms in IBD

Live CD4 T cells were sorted from inflamed and non-inflamed tissue samples of IBD patients or from healthy and IBD blood samples. ATAC-Seq libraries were generated from live CD4 T cells sorted from i) inflamed and non-inflamed tissue samples, ii) healthy and IBD blood samples, or from iii) CD4 T cell subsets polarised from healthy blood samples. After isolating crude nuclei, live CD4+ T cells were treated with Tagment DNA buffer and Tagment DNA Enzyme (Nextera DNA Library Prep Kit, Illumina), and then the DNA was purified by MinElute PCR Purification Kit (Qiagen). Transposed DNA fragments were amplified using specific adapters followed by purification with MinElute PCR Purification Kit (Qiagen). Fragments from 240-360pb were selected in the PippinHT system (Sage Science). The quality of the library and its DNA concentration were assessed by Bioanalyzer instruments (Agilent Technologies) and ultimately submitted for sequencing using Illumina HiSeq 2500 sequencer, V4 chemistry. On the other hand, single cell RNA-Seq libraries were generated exclusively from inflamed and non-inflamed tissue samples of Crohn’s disease patients. Briefly, live CD4 T cells were captured and encapsulated before cDNA amplification using the 10X Genomics Chromium Platform. Samples were prepared as outlined by 10x genomics Single Cell 3’ Reagent Kits v2 user guide. Samples were sequenced on a HiSeq 2500 with the following run parameters: Read 1 – 26 cycles, read 2 – 98 cycles, index 1 – 8 cycles.

Request Access

This is a placeholder, We had been previously advised to submit this and then send an e-mail to the helpdesk and Natalie Stickle (who coordinates out DAC) to fill out the details. She can be reached at natalie.stickle@uhn.ca. Access to data generated by UHN is made available by completing the data access agreement for review by the data access committee and will be granted to qualified investigators for appropriate use.

DATA ACCESS AGREEMENT These terms and conditions govern access to the managed access datasets (details of which are set out in Appendix I) to which the User Institution has requested access. The User Institution agrees to be bound by these terms and conditions. Definitions Authorised Personnel: The individuals at the User Institution to whom the Data Access Committee (DAC) grants access to the Data. This includes the User, the individuals listed in Appendix II and any other individuals for whom the User Institution subsequently requests access to the Data. Details of the initial Authorised Personnel are set out in Appendix II. Data: The managed access datasets to which the User Institution has requested access. Data Producers: The Principal Investigator and the collaborators listed in Appendix I responsible for the development, organisation, and oversight of these Data. External Collaborator: A collaborator of the User, working for an institution other than the User Institution. Project: The project for which the User Institution has requested access to these Data. A description of the Project is set out in Appendix II. Publications: Includes, without limitation, articles published in print journals, electronic journals, reviews, books, posters and other written and verbal presentations of research. Research Participant: An individual whose data form part of these Data. Research Purposes: Shall mean research that is seeking to advance the understanding of genetics and genomics, including the treatment of disorders, and work on statistical methods that may be applied to such research. User: The principal investigator for the Project. User Institution(s): The Institution that has requested access to the Data. University Health Network: The University Health Network (UHN) is the largest clinical and research hospital in Canada. UHN is affiliated with the University of Toronto and is a member of the Toronto Academic Health Science Network. 1. The User Institution agrees to only use these Data for the purpose of the Project (described in Appendix II) and only for Research Purposes. The User Institution further agrees that it will only use these Data for Research Purposes which are within the limitations (if any) set out in Appendix I. 2. The User Institution agrees to preserve, at all times, the confidentiality of these Data. In particular, it undertakes not to use, or attempt to use these Data to compromise or otherwise infringe the confidentiality of information on Research Participants. Without prejudice to the generality of the foregoing, the User Institution agrees to use at least the measures set out in Appendix I to protect these Data. 3. The User Institution agrees to protect the confidentiality of Research Participants in any research papers or publications that they prepare by taking all reasonable care to limit the possibility of identification. 4. The User Institution agrees not to link or combine these Data to other information or archived data available in a way that could re-identify the Research Participants, even if access to that data has been formally granted to the User Institution or is freely available without restriction. 5. The User Institution agrees only to transfer or disclose these Data, in whole or part, or any material derived from these Data, to the Authorised Personnel. Should the User Institution wish to share these Data with an External Collaborator, the External Collaborator must complete a separate application for access to these Data. 6. The User Institution agrees that the Data Producers, and all other parties involved in the creation, funding or protection of these Data: a) make no warranty or representation, express or implied as to the accuracy, quality or comprehensiveness of these Data; b) exclude to the fullest extent permitted by law all liability for actions, claims, proceedings, demands, losses (including but not limited to loss of profit), costs, awards damages and payments made by the Recipient that may arise (whether directly or indirectly) in any way whatsoever from the Recipient’s use of these Data or from the unavailability of, or break in access to, these Data for whatever reason and; c) bear no responsibility for the further analysis or interpretation of these Data. 7. The User Institution agrees to follow the Fort Lauderdale Guidelines (http://www.wellcome.ac.uk/stellent/groups/corporatesite/@policy_communications/documents/web_document/wtd003207.pdf ) and the Toronto Statement (http://www.nature.com/nature/journal/v461/n7261/full/461168a.html). This includes but is not limited to recognising the contribution of the Data Producers and including a proper acknowledgement in all reports or publications resulting from the use of these Data. 8. The User Institution agrees to follow the Publication Policy in Appendix III. This includes respecting the moratorium period for the Data Producers to publish the first peer-reviewed report describing and analysing these Data. 9. The User Institution agrees not to make intellectual property claims on these Data and not to use intellectual property protection in ways that would prevent or block access to, or use of, any element of these Data, or conclusion drawn directly from these Data. 10. The User Institution can elect to perform further research that would add intellectual and resource capital to these data and decide to obtain intellectual property rights on these downstream discoveries. In this case, the User Institution agrees to implement licensing policies that will not obstruct further research and to follow the U.S. National Institutes of Health Best Practices for the Licensing of Genomic Inventions (2005) (https://www.icgc.org/files/daco/NIH_BestPracticesLicensingGenomicInventions_2005_en.pdf ) in conformity with the Organisation for Economic Co-operation and Development Guidelines for the Licensing of the Genetic Inventions (2006) (http://www.oecd.org/science/biotech/36198812.pdf ). 11. The User Institution agrees to destroy/discard the Data held, once it is no longer used for the Project, unless obliged to retain the data for archival purposes in conformity with audit or legal requirements. 12. The User Institution will notify the DAC within 30 days of any changes or departures of Authorised Personnel. 13. The User Institution will notify the DAC prior to any significant changes to the protocol for the Project. 14. The User Institution will notify the DAC as soon as it becomes aware of a breach of the terms or conditions of this agreement. 15. The DAC may terminate this agreement by written notice to the User Institution. If this agreement terminates for any reason, the User Institution will be required to destroy any Data held, including copies and backup copies. This clause does not prevent the User Institution from retaining these data for archival purpose in conformity with audit or legal requirements. 16. The User Institution accepts that it may be necessary for the Data Producers to alter the terms of this agreement from time to time. In the event that changes are required, the Data Producers or their appointed agent will contact the User Institution to inform it of the changes and the User Institution may elect to accept the changes or terminate the agreement. 17. If requested, the User Institution will allow data security and management documentation to be inspected to verify that it is complying with the terms of this agreement. 18. The User Institution agrees to distribute a copy of these terms to the Authorised Personnel. The User Institution will procure that the Authorised Personnel comply with the terms of this agreement. 19. This agreement (and any dispute, controversy, proceedings or claim of whatever nature arising out of this agreement or its formation) shall be construed, interpreted and governed by the laws of the province of Ontario and PHIPA. Agreed for User Institution Signature: Name: Title: Date: Principal Investigator I confirm that I have read and understood this Agreement. Signature: Name: Title: Date: Agreed for The Data Access Committee Signature: Name: Title: Date: APPENDIX I – DATASET DETAILS APPENDIX II ––PROJECT DETAILS APPENDIX III –– PUBLICATION POLICY

Studies are experimental investigations of a particular phenomenon, e.g., case-control studies on a particular trait or cancer research projects reporting matching cancer normal genomes from patients.

Study ID Study Title Study Type
EGAS00001007343 Other
EGAS00001007344 Other
EGAS00001007345 Other

This table displays only public information pertaining to the files in the dataset. If you wish to access this dataset, please submit a request. If you already have access to these data files, please consult the download documentation.

ID File Type Size Located in
EGAF00008104948 fastq.gz 3.3 GB
EGAF00008104949 fastq.gz 2.5 GB
EGAF00008104950 fastq.gz 3.7 GB
EGAF00008104951 fastq.gz 2.6 GB
EGAF00008104952 fastq.gz 3.2 GB
EGAF00008104953 fastq.gz 3.6 GB
EGAF00008104954 fastq.gz 3.1 GB
EGAF00008104955 fastq.gz 2.7 GB
EGAF00008104956 fastq.gz 3.5 GB
EGAF00008104957 fastq.gz 4.1 GB
EGAF00008104958 fastq.gz 4.2 GB
EGAF00008104959 fastq.gz 3.6 GB
EGAF00008104960 fastq.gz 2.6 GB
EGAF00008104961 fastq.gz 3.0 GB
EGAF00008104962 fastq.gz 3.5 GB
EGAF00008104963 fastq.gz 2.4 GB
EGAF00008104964 fastq.gz 2.8 GB
EGAF00008104965 fastq.gz 2.5 GB
EGAF00008104966 fastq.gz 2.8 GB
EGAF00008104967 fastq.gz 2.5 GB
EGAF00008104968 fastq.gz 3.0 GB
EGAF00008104969 fastq.gz 2.2 GB
EGAF00008104970 fastq.gz 2.3 GB
EGAF00008104971 fastq.gz 4.2 GB
EGAF00008104972 fastq.gz 3.4 GB
EGAF00008104973 fastq.gz 4.2 GB
EGAF00008104974 fastq.gz 3.7 GB
EGAF00008104975 fastq.gz 3.2 GB
EGAF00008104976 fastq.gz 5.5 GB
EGAF00008104977 fastq.gz 2.7 GB
EGAF00008104978 fastq.gz 4.0 GB
EGAF00008104979 fastq.gz 5.0 GB
EGAF00008104980 fastq.gz 2.2 GB
EGAF00008104981 fastq.gz 1.6 GB
EGAF00008104982 fastq.gz 2.4 GB
EGAF00008104983 fastq.gz 2.4 GB
EGAF00008104984 fastq.gz 2.4 GB
EGAF00008104985 fastq.gz 2.8 GB
EGAF00008104986 fastq.gz 2.4 GB
EGAF00008104987 fastq.gz 2.0 GB
EGAF00008104988 fastq.gz 3.6 GB
EGAF00008104989 fastq.gz 2.2 GB
EGAF00008104990 fastq.gz 2.0 GB
EGAF00008104991 fastq.gz 2.3 GB
EGAF00008104992 fastq.gz 3.0 GB
EGAF00008104993 fastq.gz 3.3 GB
EGAF00008104994 fastq.gz 6.1 GB
EGAF00008104995 fastq.gz 2.3 GB
EGAF00008104996 fastq.gz 2.9 GB
EGAF00008104997 fastq.gz 3.1 GB
EGAF00008104998 fastq.gz 2.8 GB
EGAF00008104999 fastq.gz 5.5 GB
EGAF00008105000 fastq.gz 3.0 GB
EGAF00008105001 fastq.gz 3.6 GB
EGAF00008105002 fastq.gz 2.9 GB
EGAF00008105003 fastq.gz 3.1 GB
EGAF00008105004 fastq.gz 2.7 GB
EGAF00008105005 fastq.gz 2.4 GB
EGAF00008105006 fastq.gz 2.0 GB
EGAF00008105007 fastq.gz 3.3 GB
EGAF00008105008 fastq.gz 3.2 GB
EGAF00008105009 fastq.gz 2.8 GB
EGAF00008105010 fastq.gz 3.1 GB
EGAF00008105011 fastq.gz 2.6 GB
EGAF00008105012 fastq.gz 3.4 GB
EGAF00008105013 fastq.gz 3.8 GB
EGAF00008105014 fastq.gz 2.3 GB
EGAF00008105015 fastq.gz 3.0 GB
EGAF00008105016 fastq.gz 2.8 GB
EGAF00008105017 fastq.gz 3.0 GB
EGAF00008105018 fastq.gz 2.8 GB
EGAF00008105019 fastq.gz 3.5 GB
EGAF00008105020 fastq.gz 3.0 GB
EGAF00008105021 fastq.gz 2.0 GB
EGAF00008105022 fastq.gz 3.1 GB
EGAF00008105023 fastq.gz 2.7 GB
EGAF00008105024 fastq.gz 3.2 GB
EGAF00008105025 fastq.gz 2.7 GB
EGAF00008105026 fastq.gz 3.1 GB
EGAF00008105027 fastq.gz 3.0 GB
80 Files (244.8 GB)