Characterization of Leukemic Stem Cells in DNMT3Amut and NPM1mut AMLs

Trascriptomic profile of FACS-sorted LSCs from diagnostic DNMT3Amut and NPM1mut AML patients using a novel sorting strategy. Briefly, positive selection for GPR56 and a negative selection for NKG2DLigands were used to enrich for LSCs in both CD34+ and CD34- subpopulations. Primary samples were retrospectively collected based on their mutational profile.

Type: Other
Archiver: European Genome-Phenome Archive (EGA)

1 Dataset 1 Publication

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Dataset ID	Description	Technology	Samples
EGAD00001009293	Using a novel sorting strategy, we performed ultra low input RNAseq from FACS-sorted populations from diagnostic DNMT3Amut and NPM1mut AML patients. Primary samples were retrospectively collected based on their mutational profile. Samples were thawed, stained and FACS sorted using combination of lineage markers, CD34, GPR56 and NKG2DLigands. RNA was extracted and library prepared from 13 samples.	Illumina HiSeq 2000 NextSeq 550	7

Publications	Citations
Retained functional normal and preleukemic HSCs at diagnosis are associated with good prognosis in DNMT3AmutNPM1mut AMLs. Donato E, Correia N, Andresen C, Karpova D, Würth R, Klein C, Sohn M, Przybylla A, Zeisberger P, Rothfelder K, Salih H, Bonig H, Stasik S, Röllig C, Dolnik A, Bullinger L, Buchholz F, Thiede C, Hübschmann D, Trumpp A. Blood Adv 7: 2023 1011-1018	2