Whole genome sequencing to detect spontaneous acquired mutations in mismatch repair-deficient human colon organoids

Individual outgrowing organoids surviving the medium-based selection were manually picked and propagated as clonal lines for a couple of passages to avoid unexpected mutation accumulation before being harvested for WGS/WES. 1 µg of DNA was used for WGS/WES by Agilent SureSelect V7 kit, with 8Gb per sample (50X average mapped) data throughput, sequencing on NovaSeq 150PE

Type: Whole Genome Sequencing
Archiver: European Genome-Phenome Archive (EGA)

1 Dataset

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Dataset ID	Description	Technology	Samples
EGAD50000000159	Whole genome/exome sequencing to detect spontaneous acquired mutations in mismatch repair-deficient human colon organoids.	Illumina NovaSeq 6000 Illumina NovaSeq X	9