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Spatiotemporal immune atlas of the first clinical-grade gene-edited pig-to-human kidney xenotransplant

Pig-to-human xenotransplantation is rapidly approaching the clinical arena; however, it is unclear which immunomodulatory regimens will effectively control human immune responses to pig xenografts. We transplanted a gene-edited pig kidney into a brain-dead human recipient on pharmacologic immunosuppression and studied the human immune response to the xenograft using spatial transcriptomics and single-cell RNA sequencing. Human immune cells were uncommon in the porcine kidney cortex early after xenotransplantation and consisted of primarily myeloid cells. Both the porcine resident macrophages and human infiltrating macrophages expressed genes consistent with an alternatively activated, anti-inflammatory phenotype. No significant infiltration of human B or T cells into the porcine kidney xenograft was detected. Altogether, these findings provide proof of concept that conventional pharmacologic immunosuppression is sufficient to restrict infiltration of human immune cells into the xenograft early after compatible pig-to-human kidney xenotransplantation.

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University of Alabama at Birmingham (Xenotransplantation Project) - Data Access Policy

Access to sequence level data produced by the Porrett Lab, University of Alabama at Birmingham (Xenotransplantation Project) is controlled by Dr. Jayme Locke, and Dr. Porrett M. Paige, Principal, and Co-Principal Investigators respectively, together with members of the DAC comittee. These data were largely used in the generation of the manuscript: “Spatiotemporal immune atlas of the first clinical-grade gene-edited pig-to-human kidney xenotransplant”. Access to these data will be granted to qualified investigators for appropriate use following data use approval from members of the DAC.

Studies are experimental investigations of a particular phenomenon, e.g., case-control studies on a particular trait or cancer research projects reporting matching cancer normal genomes from patients.

Study ID Study Title Study Type
EGAS50000000244 RNASeq

This table displays only public information pertaining to the files in the dataset. If you wish to access this dataset, please submit a request. If you already have access to these data files, please consult the download documentation.

ID File Type Size Located in
EGAF50000091435 fastq.gz 1.5 GB
EGAF50000091436 fastq.gz 11.2 GB
EGAF50000091437 fastq.gz 1.2 GB
EGAF50000091438 fastq.gz 1.4 GB
EGAF50000091439 fastq.gz 1.9 GB
EGAF50000091440 fastq.gz 8.5 GB
EGAF50000091441 fastq.gz 233.5 MB
EGAF50000091442 fastq.gz 210.8 MB
EGAF50000091443 fastq.gz 201.5 MB
EGAF50000091444 fastq.gz 221.3 MB
EGAF50000091445 fastq.gz 547.0 MB
EGAF50000091446 fastq.gz 647.6 MB
EGAF50000091447 fastq.gz 204.9 MB
EGAF50000091448 fastq.gz 212.7 MB
EGAF50000091449 fastq.gz 1.1 GB
EGAF50000091450 fastq.gz 183.6 MB
EGAF50000091451 fastq.gz 324.9 MB
EGAF50000091452 fastq.gz 602.7 MB
EGAF50000091453 fastq.gz 491.6 MB
EGAF50000091454 fastq.gz 1.9 GB
EGAF50000091455 fastq.gz 10.4 GB
EGAF50000091456 fastq.gz 10.3 GB
EGAF50000091457 fastq.gz 9.3 GB
EGAF50000091458 fastq.gz 17.3 GB
EGAF50000091459 fastq.gz 181.8 MB
EGAF50000091460 fastq.gz 955.2 MB
EGAF50000091461 fastq.gz 288.1 MB
EGAF50000091462 fastq.gz 138.8 MB
EGAF50000091463 fastq.gz 13.2 GB
EGAF50000091464 fastq.gz 11.7 GB
EGAF50000091465 fastq.gz 13.9 GB
EGAF50000091466 fastq.gz 909.2 MB
EGAF50000091467 fastq.gz 144.2 MB
EGAF50000091468 fastq.gz 362.8 MB
EGAF50000091469 fastq.gz 771.4 MB
EGAF50000091470 fastq.gz 10.1 GB
36 Files (132.9 GB)